Review



anti p tau s404  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Cell Signaling Technology Inc anti p tau s404
    Anti P Tau S404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12910249-44-141-143
    Average 95 stars, based on 79 article reviews
    anti p tau s404 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Dietary salt promotes cognition impairment through GLP-1R/mTOR/p70S6K signaling pathway
    Article Snippet: .. Subsequently, the proteins on the gels were transferred to PVDF membranes (Bio-Rad, Hercules, USA), then blocked with TBST containing 5% milk for 2 h, and incubated at 4 °C overnight with the following primary antibodies: rabbit anti-GLP-1R (1:500, cat.97308, Novus Biologicals, USA), rabbit anti-beclin1 (1:1000, cat. #3495, Cell Signaling Technology, USA), rabbit anti-SQSTM1/p62 (1:1000, cat.#23214, Cell Signaling Technology, USA), rabbit anti-LC3A/B (1:1000, cat.#12741, Cell Signaling Technology, USA), rabbit anti-mTOR(1:1000, cat.#2983, Cell Signaling Technology, USA), rabbit anti-phospho-mTOR (Ser2448, 1:1000, cat.#5536, Cell Signaling Technology, USA), rabbit anti-p70S6 Kinase (1:1000, cat.#34475, Cell Signaling Technology, USA), rabbit anti-phospho-p70S6 Kinase (1:1000, cat.#9234,Cell Signaling Technology, USA), rabbit anti-PSD95 (1:1000, Cell Signaling Technology, USA), Mouse anti-SYP (1:2000, sc-17750, Santa Cruz, USA), rabbit anti-MAP2 (1:500, bs-1369R, Bioss Biotechnology Co., Ltd, China), mouse anti-Tau46 (1:1000, cat. #4019, Cell Signaling Technology); rabbit anti-phospho-tau Thr205 (1:1000, cat. #49561, Cell Signaling Technology, USA); rabbit anti-phospho-tau Thr181 (1:1000, cat. #12885, Cell Signaling Technology, USA); rabbit anti-phospho-tau Ser404 (1:1000, cat. #20194; Cell Signaling Technology, USA); mouse anti-GAPDH (1:1000, cat. AF0006, Beyotime Biotechnology); mouse anti-β-actin (1:1000; cat. AF5003; Beyotime Biotechnology). .. Then the membranes were shocked with TBST (3 × 10 min) and incubated with secondary antibodies (anti-mouse IgG or anti-rabbit IgG, Beyotime Biotechnology) at room temperature for 1 h. Ultimately, an ECL chemiluminescent system (Tanon-5200Multi, Shanghai, China) was used to expose the aim protein, and ImageJ 1.8.0 software (NIH) was employed for calculating the gray value of the target protein bands.

    Article Title: Neuronal Dynamics and miRNA Signaling Differ between SH-SY5Y APPSwe and PSEN1 Mutant iPSC-Derived AD Models upon Modulation with miR-124 Mimic and Inhibitor
    Article Snippet: Samples (30 μg of exosomes, 40 μg of SH- WT or SH- SWE cells and 20 μg of iNEU- WT and iNEU- PSEN cells) were separated on Tris-Tricine gel, transferred into nitrocellulose membranes (Amersham, Health, Buckinghamshire, United Kingdom) and incubated in blocking buffer (5% ( w / v ) non-fat dried milk in Tween 20 (0.1%) tween-tris buffer saline (T-TBS)) for 1 h at room temperature. .. Membranes were incubated at 4 °C overnight with primary antibodies: mouse anti-ALIX (1:1000, Cell Signaling); goat anti-CD63 (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-flotilin-1 (1:1000, BD Biosciences, Franklin Lakes, NJ, USA); mouse anti-amyloid β (1:1000, Merck Millipore, Burlington, MA, USA); rabbit anti-phospho-Tau (Ser404) (1:1000, Cell Signaling); rabbit anti-Tau (1:1000, Synaptic Systems, Goettingen, Germany); and mouse anti-β-actin (1:2000, Sigma Aldrich, St. Louis, MO, USA). .. Each antibody was diluted in blocking buffer, followed by incubation with the respective secondary antibodies for 1 h at room temperature, using HRP-conjugated goat anti-mouse (1:2000), rabbit anti-goat (1:2000), and goat-anti-rabbit (1:2000), all from Santa Cruz Biotechnology.

    Article Title: Multimodal MRI combined with RNA sequencing reveals pathological signatures in the 9-month-old 3×Tg-AD mouse brain
    Article Snippet: .. Sections were then incubated with primary antibodies: mouse anti-Aβ clone 6E10 (1:1000, Cat# 803001, Biolegend, Dedham, MA, USA) and rabbit anti-phospho-Tau (Ser404) (1:1000, Cat# 2019s, CST, Danvers, MA, USA) at 4°C overnight. .. After primary antibody incubation, sections were washed and incubated for 2 hours at room temperature in the dark with corresponding secondary antibodies including goat antimouse Alexa Fluor 488 (1:1000, Cat# ab150113, Abcam, Cambridge, MA, USA) and goat anti-rabbit Alexa Fluor 488 (1:1000, Cat# ab150077, Abcam).

    Translocation Assay:

    Article Title: Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice.
    Article Snippet: Taken together, our study demonstrates TMEM106B loss of function strongly modifies tauopathy and its associated phenotypes, such as neuroinflammation, disruption of neuronal cytoskeleton, autophagy-lysosomal defects, and subsequent neurodegenerative phenotypes, and provides new insights into the pathophysiological functions of TMEM106B in the brain. .. The following antibodies were used in this study: mouse anti-GAPDH (Proteintech Group, 60004-1-Ig), rat antihuman Tau (BioLegend, A16103A), rabbit anti-Phospho-Tau (Ser404) (Cell signaling, 20194S), rabbit anti-Phospho-Tau (Thr205) (Cell signaling, 49561S), mouse anti-Tau (Proteintech Group, 66499-1-Ig), mouse anti-NeuN (Millipore, MAB377), rabbit anti-NeuN (GeneTex, GTX132974), rabbit anti-Cleaved Caspase-3 (Asp175) (Cell signaling, 9661), mouse anti-β-III-tubulin (Promega, G7121), mouse antiacetylated Tubulin (Lys40) (Proteintech Group, 66200-1-Ig), rabbit anti-alpha-tubulin (Proteintech Group, 11224-1-AP), Fig. 8 TMEM106B deficiency increases TFE3 nuclear translocation and Galectin-3 upregulation in microglia in PS19 mice. a, b Representative images of CathD and IBA1 co-immunostaining in hippocampal CA3 region of 8.5-month-old PS19 and Tmem106b−/− PS19 mice. ..



    Similar Products

    95
    Cell Signaling Technology Inc anti p tau s404
    Anti P Tau S404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12910249-44-141-143
    Average 95 stars, based on 1 article reviews
    anti p tau s404 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc p tau ser404
    CFE downregulated hippocampal levels of Aβ1–42, GSK-3β, Tau and P-Tau in HNF mice. The colored dots represent data points from different sample groups. ( A ) Representative immunohistochemical (IHC) staining images of Aβ1–42 (40× magnification). ( B ) Quantification of Aβ1–42 IHC staining intensity expressed as average optical density (AOD). ( C ) Representative Western blot images of hippocampal Aβ1–42. ( D ) Quantitative analysis of Aβ1–42 protein levels from Western blot, normalized to GAPDH. ( E ) Representative IHC staining images of total Tau and P-Tau <t>(Ser404)</t> (40×). ( F , G ) Quantification of Tau and P-Tau (Ser404) IHC staining intensity (AOD). ( H ) Representative Western blot images of GSK-3β, total Tau and P-Tau (Ser404). ( I – K ) Quantitative analysis of GSK-3β, total Tau and P-Tau (Ser404) protein levels from Western blot, normalized to GAPDH. Data are presented as the mean ± SEM (N = 3). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05, compared to the LPS group.
    P Tau Ser404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12736303-194-31-35
    Average 93 stars, based on 1 article reviews
    p tau ser404 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti phosphotau ser404
    CFE downregulated hippocampal levels of Aβ1–42, GSK-3β, Tau and P-Tau in HNF mice. The colored dots represent data points from different sample groups. ( A ) Representative immunohistochemical (IHC) staining images of Aβ1–42 (40× magnification). ( B ) Quantification of Aβ1–42 IHC staining intensity expressed as average optical density (AOD). ( C ) Representative Western blot images of hippocampal Aβ1–42. ( D ) Quantitative analysis of Aβ1–42 protein levels from Western blot, normalized to GAPDH. ( E ) Representative IHC staining images of total Tau and P-Tau <t>(Ser404)</t> (40×). ( F , G ) Quantification of Tau and P-Tau (Ser404) IHC staining intensity (AOD). ( H ) Representative Western blot images of GSK-3β, total Tau and P-Tau (Ser404). ( I – K ) Quantitative analysis of GSK-3β, total Tau and P-Tau (Ser404) protein levels from Western blot, normalized to GAPDH. Data are presented as the mean ± SEM (N = 3). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05, compared to the LPS group.
    Anti Phosphotau Ser404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pm41199342-319-30-35
    Average 95 stars, based on 1 article reviews
    anti phosphotau ser404 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti phospho tau ser404
    CFE downregulated hippocampal levels of Aβ1–42, GSK-3β, Tau and P-Tau in HNF mice. The colored dots represent data points from different sample groups. ( A ) Representative immunohistochemical (IHC) staining images of Aβ1–42 (40× magnification). ( B ) Quantification of Aβ1–42 IHC staining intensity expressed as average optical density (AOD). ( C ) Representative Western blot images of hippocampal Aβ1–42. ( D ) Quantitative analysis of Aβ1–42 protein levels from Western blot, normalized to GAPDH. ( E ) Representative IHC staining images of total Tau and P-Tau <t>(Ser404)</t> (40×). ( F , G ) Quantification of Tau and P-Tau (Ser404) IHC staining intensity (AOD). ( H ) Representative Western blot images of GSK-3β, total Tau and P-Tau (Ser404). ( I – K ) Quantitative analysis of GSK-3β, total Tau and P-Tau (Ser404) protein levels from Western blot, normalized to GAPDH. Data are presented as the mean ± SEM (N = 3). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05, compared to the LPS group.
    Anti Phospho Tau Ser404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12590737-353-30-35
    Average 95 stars, based on 1 article reviews
    anti phospho tau ser404 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti tau ps404
    Sorafenib treatment reduces tau levels in vitro . (A, B) SH-SY5Y tau(P301L)-EGFP cells were treated with 54 FDA-approved drugs (10 μmol/L) or DMSO control for 24 h. Protein levels of total tau (A) and phosphorylated tau <t>pS404</t> (B) were analyzed by immunoblotting, and their levels relative to those of controls were determined. (C) HEK293 cells stably expressing tau (HEK293-tau) were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were subjected to a single-molecule immunoassay to determine phosphorylated tau pT181, pT217, and pT231 levels. n = 4. Paired Student's t -test. (D, E) SH-SY5Y tau(P301L)-EGFP cells were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were analyzed by immunoblotting for total tau and phosphorylated tau, including pT181, pT212, pT231, and pS396 (D). Protein levels were quantified by densitometry for comparison (E). n = 3. Unpaired Student's t -test. (F, G) Wild-type (WT) primary neurons derived from C57BL6/J mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (F). Total tau levels were quantified for comparison (G). n = 3. Unpaired Student's t -test. (H, I) Primary neurons derived from PS19 mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (H). Protein levels of total tau and phosphorylated tau (pT231 and pS396) were quantified for comparison (I). n = 5. Unpaired Student's t -test. Data are presented as mean ± SEM; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001.
    Anti Tau Ps404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12648003-38-62-65
    Average 95 stars, based on 1 article reviews
    anti tau ps404 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti ps404
    Sorafenib treatment reduces tau levels in vitro . (A, B) SH-SY5Y tau(P301L)-EGFP cells were treated with 54 FDA-approved drugs (10 μmol/L) or DMSO control for 24 h. Protein levels of total tau (A) and phosphorylated tau <t>pS404</t> (B) were analyzed by immunoblotting, and their levels relative to those of controls were determined. (C) HEK293 cells stably expressing tau (HEK293-tau) were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were subjected to a single-molecule immunoassay to determine phosphorylated tau pT181, pT217, and pT231 levels. n = 4. Paired Student's t -test. (D, E) SH-SY5Y tau(P301L)-EGFP cells were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were analyzed by immunoblotting for total tau and phosphorylated tau, including pT181, pT212, pT231, and pS396 (D). Protein levels were quantified by densitometry for comparison (E). n = 3. Unpaired Student's t -test. (F, G) Wild-type (WT) primary neurons derived from C57BL6/J mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (F). Total tau levels were quantified for comparison (G). n = 3. Unpaired Student's t -test. (H, I) Primary neurons derived from PS19 mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (H). Protein levels of total tau and phosphorylated tau (pT231 and pS396) were quantified for comparison (I). n = 5. Unpaired Student's t -test. Data are presented as mean ± SEM; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001.
    Anti Ps404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pm41106693-45-24-25
    Average 95 stars, based on 1 article reviews
    anti ps404 - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc rabbit anti phospho tau ser404
    Sorafenib treatment reduces tau levels in vitro . (A, B) SH-SY5Y tau(P301L)-EGFP cells were treated with 54 FDA-approved drugs (10 μmol/L) or DMSO control for 24 h. Protein levels of total tau (A) and phosphorylated tau <t>pS404</t> (B) were analyzed by immunoblotting, and their levels relative to those of controls were determined. (C) HEK293 cells stably expressing tau (HEK293-tau) were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were subjected to a single-molecule immunoassay to determine phosphorylated tau pT181, pT217, and pT231 levels. n = 4. Paired Student's t -test. (D, E) SH-SY5Y tau(P301L)-EGFP cells were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were analyzed by immunoblotting for total tau and phosphorylated tau, including pT181, pT212, pT231, and pS396 (D). Protein levels were quantified by densitometry for comparison (E). n = 3. Unpaired Student's t -test. (F, G) Wild-type (WT) primary neurons derived from C57BL6/J mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (F). Total tau levels were quantified for comparison (G). n = 3. Unpaired Student's t -test. (H, I) Primary neurons derived from PS19 mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (H). Protein levels of total tau and phosphorylated tau (pT231 and pS396) were quantified for comparison (I). n = 5. Unpaired Student's t -test. Data are presented as mean ± SEM; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001.
    Rabbit Anti Phospho Tau Ser404, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/FoxD3+Rabbit+mAb/10__4103_slash_nrr__nrr___d___25___00006-153-19-25
    Average 96 stars, based on 1 article reviews
    rabbit anti phospho tau ser404 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc phospho tau ser404 d2z4g rabbit mab
    GBP treatment caused tau hyperphosphorylation in the hippocampus of aged mice. (A) Using Phospho-Tau Family Antibody Sampler Kit, the levels of total tau protein and phosphorylated tau at five sites were analyzed using Western blot analysis. (B,C) The levels of <t>p-tau</t> (S262) and p-tau (S416) were increased in GBP-treated sham and SNI mice (*P < 0.05, **P < 0.01, n = 4/group). (D–G) The levels of p-tau (S202), p-tau (S396), p-tau (S404), or total tau protein did not significantly differ between GBP- and saline-treated mice.
    Phospho Tau Ser404 D2z4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+tau+ser404/Phospho-Tau+(Ser404)+Rabbit+mAb/pmc12440869-5-0-6
    Average 95 stars, based on 1 article reviews
    phospho tau ser404 d2z4g rabbit mab - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    CFE downregulated hippocampal levels of Aβ1–42, GSK-3β, Tau and P-Tau in HNF mice. The colored dots represent data points from different sample groups. ( A ) Representative immunohistochemical (IHC) staining images of Aβ1–42 (40× magnification). ( B ) Quantification of Aβ1–42 IHC staining intensity expressed as average optical density (AOD). ( C ) Representative Western blot images of hippocampal Aβ1–42. ( D ) Quantitative analysis of Aβ1–42 protein levels from Western blot, normalized to GAPDH. ( E ) Representative IHC staining images of total Tau and P-Tau (Ser404) (40×). ( F , G ) Quantification of Tau and P-Tau (Ser404) IHC staining intensity (AOD). ( H ) Representative Western blot images of GSK-3β, total Tau and P-Tau (Ser404). ( I – K ) Quantitative analysis of GSK-3β, total Tau and P-Tau (Ser404) protein levels from Western blot, normalized to GAPDH. Data are presented as the mean ± SEM (N = 3). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05, compared to the LPS group.

    Journal: Nutrients

    Article Title: From Microbiota to Metabolomics: How Corylus heterophylla Fisch. Male Flower Extract Shields Mice from Cognitive Decline

    doi: 10.3390/nu17243958

    Figure Lengend Snippet: CFE downregulated hippocampal levels of Aβ1–42, GSK-3β, Tau and P-Tau in HNF mice. The colored dots represent data points from different sample groups. ( A ) Representative immunohistochemical (IHC) staining images of Aβ1–42 (40× magnification). ( B ) Quantification of Aβ1–42 IHC staining intensity expressed as average optical density (AOD). ( C ) Representative Western blot images of hippocampal Aβ1–42. ( D ) Quantitative analysis of Aβ1–42 protein levels from Western blot, normalized to GAPDH. ( E ) Representative IHC staining images of total Tau and P-Tau (Ser404) (40×). ( F , G ) Quantification of Tau and P-Tau (Ser404) IHC staining intensity (AOD). ( H ) Representative Western blot images of GSK-3β, total Tau and P-Tau (Ser404). ( I – K ) Quantitative analysis of GSK-3β, total Tau and P-Tau (Ser404) protein levels from Western blot, normalized to GAPDH. Data are presented as the mean ± SEM (N = 3). Statistically significant differences were indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05, compared to the LPS group.

    Article Snippet: IHC staining of brain tissue sections was performed using primary antibodies against the following targets: Aβ1–42 (cat. #A9810, Sigma Aldrich, St. Louis, MO, USA), Tau (cat. #10274-1-AP, Proteintech, Rosemont, IL, USA), P-Tau (Ser404) (cat. #35834, Cell Signaling Technology, Danvers, MA, USA), BDNF (cat. #25699-1-AP, Proteintech), MAP2 (cat. #17490-1-AP, Proteintech) and CD11b (cat. #66519-1-IG, Proteintech).

    Techniques: Immunohistochemical staining, Immunohistochemistry, Western Blot

    Sorafenib treatment reduces tau levels in vitro . (A, B) SH-SY5Y tau(P301L)-EGFP cells were treated with 54 FDA-approved drugs (10 μmol/L) or DMSO control for 24 h. Protein levels of total tau (A) and phosphorylated tau pS404 (B) were analyzed by immunoblotting, and their levels relative to those of controls were determined. (C) HEK293 cells stably expressing tau (HEK293-tau) were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were subjected to a single-molecule immunoassay to determine phosphorylated tau pT181, pT217, and pT231 levels. n = 4. Paired Student's t -test. (D, E) SH-SY5Y tau(P301L)-EGFP cells were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were analyzed by immunoblotting for total tau and phosphorylated tau, including pT181, pT212, pT231, and pS396 (D). Protein levels were quantified by densitometry for comparison (E). n = 3. Unpaired Student's t -test. (F, G) Wild-type (WT) primary neurons derived from C57BL6/J mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (F). Total tau levels were quantified for comparison (G). n = 3. Unpaired Student's t -test. (H, I) Primary neurons derived from PS19 mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (H). Protein levels of total tau and phosphorylated tau (pT231 and pS396) were quantified for comparison (I). n = 5. Unpaired Student's t -test. Data are presented as mean ± SEM; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Sorafenib promotes the E3 ubiquitin ligase FBXW7 to increase tau degradation and ameliorate tauopathies

    doi: 10.1016/j.apsb.2025.09.024

    Figure Lengend Snippet: Sorafenib treatment reduces tau levels in vitro . (A, B) SH-SY5Y tau(P301L)-EGFP cells were treated with 54 FDA-approved drugs (10 μmol/L) or DMSO control for 24 h. Protein levels of total tau (A) and phosphorylated tau pS404 (B) were analyzed by immunoblotting, and their levels relative to those of controls were determined. (C) HEK293 cells stably expressing tau (HEK293-tau) were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were subjected to a single-molecule immunoassay to determine phosphorylated tau pT181, pT217, and pT231 levels. n = 4. Paired Student's t -test. (D, E) SH-SY5Y tau(P301L)-EGFP cells were treated with sorafenib (10 μmol/L) or DMSO control for 24 h. Equal amounts of protein lysates were analyzed by immunoblotting for total tau and phosphorylated tau, including pT181, pT212, pT231, and pS396 (D). Protein levels were quantified by densitometry for comparison (E). n = 3. Unpaired Student's t -test. (F, G) Wild-type (WT) primary neurons derived from C57BL6/J mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (F). Total tau levels were quantified for comparison (G). n = 3. Unpaired Student's t -test. (H, I) Primary neurons derived from PS19 mice were treated with sorafenib (10 μmol/L) or DMSO control for 12 h. Equal amounts of protein lysates were analyzed by immunoblotting (H). Protein levels of total tau and phosphorylated tau (pT231 and pS396) were quantified for comparison (I). n = 5. Unpaired Student's t -test. Data are presented as mean ± SEM; ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001.

    Article Snippet: Antibodies used in the present study including anti-FBXW7 (A5872, Abclonal, Wuhan, China), anti-FBXW7 (55290-1-AP, Proteintech, Wuhan, China), anti-total tau (tau5; AHB0042, Thermo Fisher Scientific), anti-tau pS199 (44734G, Thermo Fisher Scientific), anti-tau pS202/T205 (AT8; MN1020, Thermo Fisher Scientific), anti-tau pS396 (44752G, Thermo Fisher Scientific), anti-tau pT231 (A19710, Abclonal), anti-tau pT231/S235) (20473, Cell Signaling Technology, Danvers, MA, USA), anti-tau pT181 (12885, Cell Signaling Technology), anti-tau pS404 (20194, Cell Signaling Technology), anti-tau pS202 (11834, Cell Signaling Technology), anti-tau pS235 ( R25855 , Zen Bio, Chengdu, China), anti-LC3 (3868S, Cell Signaling Technology), anti-mTOR (2983, Cell Signaling Technology), anti-p-mTOR (Ser2448) (5536, Cell Signaling Technology), anti-S6 (2217, Cell Signaling Technology), anti-pS6 (5364, Cell Signaling Technology), anti-ERK1/2 (4695, Cell Signaling Technology), anti-pERK1/2 (4370, Cell Signaling Technology), anti-GSK3 β (51065-1-AP, Proteintech), anti-pGSK3 β (Y216, Y279) (44604G, Thermo Fisher Scientific), anti-p38 (9212, Cell Signaling Technology), anti-p-p38 (4511, Cell Signaling Technology), anti-CDK5 (sc-173, Santa Cruz Biotechnology, Dallas, TX, USA), anti-GFP (50430-2-AP, Proteintech), anti-flag (20543-1-AP, Proteintech), anti-flag (66008-4-Ig, Proteintech), anti-myc (2276S, Cell Signaling Technology), anti-myc (16286-1-AP, Proteintech), anti-HA (51064-2-AP, Proteintech), anti-Ub (PTM-1106RM, PTM biolabs, Hangzhou, China), anti-GFAP (3670, Cell Signaling Technology), anti-Tuj1 (sc-173, Biolegend, San Diego, CA, USA), anti-NeuN (94403, Cell Signaling Technology), anti-Iba1 (17198, Cell Signaling Technology), anti-GAPDH (ab0038, Abways, Shanghai, China), and anti- β -actin (AC026, Abclonal).

    Techniques: In Vitro, Control, Western Blot, Stable Transfection, Expressing, Comparison, Derivative Assay

    GBP treatment caused tau hyperphosphorylation in the hippocampus of aged mice. (A) Using Phospho-Tau Family Antibody Sampler Kit, the levels of total tau protein and phosphorylated tau at five sites were analyzed using Western blot analysis. (B,C) The levels of p-tau (S262) and p-tau (S416) were increased in GBP-treated sham and SNI mice (*P < 0.05, **P < 0.01, n = 4/group). (D–G) The levels of p-tau (S202), p-tau (S396), p-tau (S404), or total tau protein did not significantly differ between GBP- and saline-treated mice.

    Journal: Frontiers in Pharmacology

    Article Title: Long-term gabapentin treatment impairs cognitive function in aged mice via tau hyperphosphorylation

    doi: 10.3389/fphar.2025.1616775

    Figure Lengend Snippet: GBP treatment caused tau hyperphosphorylation in the hippocampus of aged mice. (A) Using Phospho-Tau Family Antibody Sampler Kit, the levels of total tau protein and phosphorylated tau at five sites were analyzed using Western blot analysis. (B,C) The levels of p-tau (S262) and p-tau (S416) were increased in GBP-treated sham and SNI mice (*P < 0.05, **P < 0.01, n = 4/group). (D–G) The levels of p-tau (S202), p-tau (S396), p-tau (S404), or total tau protein did not significantly differ between GBP- and saline-treated mice.

    Article Snippet: Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb , Cell Signaling , 20194 , 1:1000.

    Techniques: Western Blot, Saline

    AAV-mediated Sirt1 overexpression in the hippocampus inhibited CaMKIIα expression and tau hyperphosphorylation in GBP-treated SNI aged mice. (A) Sirt1 overexpression restored Sirt1 activity in GBP-treated mice. (B) Western blot images of Sirt1, CaMKIIα, and tau proteins. (C) In eGFP expressing mice, Sirt1 protein levels were decreased by GBP treatment. (D,E,F) Overexpression of Sirt1 inhibited CaMKIIα protein levels, phosphorylation of p-tau (S416) and p-tau (216) in GBP-treated mice. (G) Total tau level was not affected by Sirt1 overexpression (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4/group).

    Journal: Frontiers in Pharmacology

    Article Title: Long-term gabapentin treatment impairs cognitive function in aged mice via tau hyperphosphorylation

    doi: 10.3389/fphar.2025.1616775

    Figure Lengend Snippet: AAV-mediated Sirt1 overexpression in the hippocampus inhibited CaMKIIα expression and tau hyperphosphorylation in GBP-treated SNI aged mice. (A) Sirt1 overexpression restored Sirt1 activity in GBP-treated mice. (B) Western blot images of Sirt1, CaMKIIα, and tau proteins. (C) In eGFP expressing mice, Sirt1 protein levels were decreased by GBP treatment. (D,E,F) Overexpression of Sirt1 inhibited CaMKIIα protein levels, phosphorylation of p-tau (S416) and p-tau (216) in GBP-treated mice. (G) Total tau level was not affected by Sirt1 overexpression (*p < 0.05, **p < 0.01, ***p < 0.001, n = 4/group).

    Article Snippet: Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb , Cell Signaling , 20194 , 1:1000.

    Techniques: Over Expression, Expressing, Activity Assay, Western Blot, Phospho-proteomics

    Resveratrol treatment boosted Sirt1 activity and inhibited CaMKIIα expression and tau hyperphosphorylation in GBP-treated aged SNI mice. (A) GBP treatment inhibited Sirt1 activity and RSV treatment restored Sirt1 activity in mice. (B) Western blot of CaMKIIα and tau proteins. (C–F) Activation of Sirt1 suppressed CaMKIIα expression, phosphorylation of p-tau (S262) and p-tau (416) in GBP-treated mice. Total tau level was not affected by resveratrol treatment. (*p < 0.05, **p < 0.01, n = 4/group).

    Journal: Frontiers in Pharmacology

    Article Title: Long-term gabapentin treatment impairs cognitive function in aged mice via tau hyperphosphorylation

    doi: 10.3389/fphar.2025.1616775

    Figure Lengend Snippet: Resveratrol treatment boosted Sirt1 activity and inhibited CaMKIIα expression and tau hyperphosphorylation in GBP-treated aged SNI mice. (A) GBP treatment inhibited Sirt1 activity and RSV treatment restored Sirt1 activity in mice. (B) Western blot of CaMKIIα and tau proteins. (C–F) Activation of Sirt1 suppressed CaMKIIα expression, phosphorylation of p-tau (S262) and p-tau (416) in GBP-treated mice. Total tau level was not affected by resveratrol treatment. (*p < 0.05, **p < 0.01, n = 4/group).

    Article Snippet: Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb , Cell Signaling , 20194 , 1:1000.

    Techniques: Activity Assay, Expressing, Western Blot, Activation Assay, Phospho-proteomics